Overview

This is a work in progress of the analysis of Erika’s cellular response data.

Presented here are the all identified DNCB modified peptides in HaCaTs, and a comparison of the observed protein concentration for DNCB treatment at 24 and 48 hours versus Control at the start of the assay.

Click here to download the csv files.

DNCB modified peptide tables

Hopefully the columns are self-explanatory, but the exp column is the experiment in which the peptides was observed, and the peptide.modification contains the position in peptide.seq at which the modification occurs. A * means the position couldn’t be confirmed.

1 hour timepoint

2 hour timepoint

4 hour timepoint

8 hour timepoint

24 hour timepoint

48 hour timepoint

Control versus DNCB treatment protein abundance

Fold change for fmol of protein observed is on log2 scale meaning that each fold change increment of 1 represents double the concentration.

The p-value on a log10 scale means that a p-value of 0.05 or below is transformed to 1.3 or above and a p-value of 0.01 is equal to 2.

Volcano plot 24 hours

Table of significantly different proteins at 24 hours

Volcano plot 48 hours

Table of significantly different proteins at 48 hours

Gene ontology of significantly different proteins at 24 hours

No significant terms were found for the 48 hour time point.

Modified amino acids

The relative proportions of the modified amino acid of all modified peptides in all experiments and all timepoints.

Counts

Percentage